Prakash Parajuli

9 Questions 13 Answers 0 Followers

Questions related from Prakash Parajuli

Is there any way to control the mutarotation of sugars (D-glucose, D-maltose, etc) in aqueous solution?

Any condition available to use specific anomeric sugar for reaction? Example; D-glucose exists as a mixture of 36% α- and 64% β- How can I selectively use 36% α- only or 64% β- for further...

23 March 2017 7,769 2 View

Why the sugar conjugation is in alpha-configuration even though the sugar donor is in alpha-form in case of inverting glycosyltransferase?

In case of inverting glycosyltransferase, when I use D-alpha-sugar as donor substrate than the sugar conjugation to substrate is in beta-configuration but when I use L-alpha-sugar as donor than I...

20 September 2016 8,207 1 View

Is UDP/TDP-beta-D-glucose commercially available?

Is it possible to buy UDP/TDP-beta-D-glucose from any commercial source with reasonable price?

31 August 2016 4,415 4 View

Is it possible to get alpha-linkage and beta-linkage glucoside product using (single) same glycosyltransferase?

Is it possible that a single glycosyltransferase can attach sugar in alpha-linkage and beta-linkage over same compound? For example; beta-linkage glucoside with D-glucose but alpha-linkage...

13 July 2016 5,703 4 View

Is it possible to have doublet of doublet spectrums in anomeric proton of polyphenol glycosides?

I am wondering, if it is possible to have multiplate and doublet of doublet anomeric protons in polyphenol glycosides? If possible then in what case? Usually, alpha- and beta- configurations of...

18 May 2016 4,769 6 View

Is it possible to have multiplate spectrums instead of doblet in anomeric proton of Polyphenol glycosides?

It is obvious to get doblet spectrum of anomeric proton while analyzing 1H NMR of phenolic glycosides. What if I get multiplate spectrums in anomeric region and also present of sugar protons in...

16 March 2016 3,663 2 View

I am curious to know the effect on E. coli BL21 (DE3) cell if I completely knockdown the glucose 1-phosphate thymidylyltransferase(tgs, rfbA or rffH)?

Is there is any possibility of the formation of TDP-rhmanose sugar in E. coli BL21 (DE3) if the glucose-1-phosphate thymidylyltransferase is knockdown? I appreciate the experts opinion and...

12 January 2016 4,673 2 View

The presence of UDP-D-glucose and TDP-L-rhamnose in E. coli BL21 (DE3) are in alpha- or beta- configuration?

I am curious to know the indigenous NDP-sugars(UDP-D-glucose and TDP-L-rhamnose) in E. coli BL21 (DE3) are in alpha- or beta- configuration. Since it is important to know for the sterio-selective...

16 October 2015 1,725 4 View

Does single glycosyltransferase has inversion as well as retention property?

Is it possible a single glycosyltransefrase can have inversion as well as retention properties with a single acceptor substrate? Are there any published references?

01 January 1970 9,400 4 View