Prafull Kumar Singh

7 Questions 26 Answers 0 Followers

Questions related from Prafull Kumar Singh

Transient expression of a gene by using just the retroviral transfer plasmid possible?

If I transfect my cells just with the retroviral transfer plasmid ( pWZL- Hygro) without the packaging vector (pCl-eco), would it only be transiently expressed? The retroviral vector sequence...

13 February 2020 8,644 3 View

Which is the best detergent for studying protein interaction by co-IP experiments?

I am interested in studying the interaction of outer mitochondrial membrane bound proteins by co-immunoprecipitation. Which would be the most suitable detergent for this study: Digitonin Vs CHAPS...

20 August 2016 1,756 3 View

Are there Prestained Marker available for Native Gel to enable monitoring during electrophoresis?

Hi Everyone I am doing Native Gel electrophoresis for quite some time and have been using own made protein standards to get an idea about the size of my target proteins. These markers are...

01 April 2016 5,409 3 View

Are dephosphorylated single stranded overhangs substrate for Mung Bean Nuclease?

I want to get rid of a restriction site for an enzyme in my vector. At first I tried to mutate the site by site directed mutagenesis but somehow it did not work. Then I adapted a straight forward...

02 October 2015 9,268 3 View

What is the difference between absorption and Excitation spectra?

I am performing cellular uptake studies with peptides labelled with fluorochromes. I did flow cytometry experiments and now I want to validate it by fluorimetry by measuring the emission spectrum...

12 March 2014 9,293 4 View

Does anyone have experience with competition assay with labeled and unlabeled peptide?

I am working on development of cell type specific delivery peptides. To prove the receptor specificity of these peptides, I am performing competition assay between Fluorochrome labelled and...

08 February 2014 5,909 8 View

Any insight as to Flow Cytometry vs Fluorescence Microscopy?

I am performing experiments for cellular binding of specific peptides. These peptides are labelled with FITC. For some peptides, I do not see any fluorescence in the microscope, however and flow...

05 February 2014 2,577 2 View