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I have a protein of interest that is expressing in Pichia. It is expressing well in the shaker flask, but when I apply the same expression methodology but now in a bioreactor I get minimal...
04 April 2018 6,925 8 View
Procedure: Split supernatent into three individual samples A, B, C and do 20% NH4SO4 cut on sample A, 40% cut on sample B, and 60% cut on sample C. Add granulated ammonium sulfate NOT a saturated...
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According to Invitrogen pichia pastoris cloning system using pPIC vectors there is an option to insert into AOX1 or HIS4 when using GS115 or KM71 strains. In both cases the SAME phenotype is...
10 October 2017 5,194 2 View
Structurally they are similar and my understanding is that the OH groups are oxidized which ultimately modulates fluorescence. Both of these molecules are identical in that respect so why is DCFDA...
05 May 2016 541 2 View
In particular what biosynthetic sources exist. Glutamate can come from food sources as well as from glutamine so let's check those off the list. Can glutamate come from alpha ketoglutarte...
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The old school way (plasmid/homologous recombination) not using CRISPR. I can't find anything relevant right now. Does anyone have a PDF or some manuscript describing a step by step sequence? It...
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