Pfano Mbedzi

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Questions related from Pfano Mbedzi

Can I sequence SSR marker for soybean if I only see one band on a agarose gel?

I am doing some SSR work on soybean, my understanding is that as a tetraploid, soybean can have either one of the four alternative alleles in one locus for SSR (Simple sequence repeat) markers? (I...

06 September 2016 8,760 2 View

Is it possible to get different SSR allele size on individuals of the same cultivar?

I looked at allele size of SSR markers on williams 82 and compared it to the size on the same cultivar that were published on SoyBase. my question is, even though both are williams cultivar (the...

01 January 1970 2,698 10 View

What is the meaning of double bands on SSR marker PCR product?

I did a normal single marker PCR for my SSR markers and analysed the PCR product on a 3% agarose gel. two of the markers show two bands on the gel, the bands are very close together but there is...

01 January 1970 1,978 10 View

How long can I keep lyophilized primers before re-suspending?

I received fluorescent labelled primers and I am not sure how to re-suspend them. The information sheet doesn't give instructions that are usually given with primers, so I decide to wait until the...

01 January 1970 4,942 10 View