Pfano Mbedzi

6 Questions 8 Answers 0 Followers

Questions related from Pfano Mbedzi

What is Difference between Co-variance standardized and distance standardized measures of PCoA?

Hi When constructing a principal coordinate analysis (PCoA) to see (dis)similarity in a set of data, you have to choose between "Co-variance standardized" and "distance standardized" measures of...

22 June 2017 4,168 1 View

Can I sequence SSR marker for soybean if I only see one band on a agarose gel?

I am doing some SSR work on soybean, my understanding is that as a tetraploid, soybean can have either one of the four alternative alleles in one locus for SSR (Simple sequence repeat) markers? (I...

06 September 2016 8,778 2 View

How to store sclerotia from Sclerotinia sclerotiorum for long term?

I am trying to find out how to store sclerotia from Sclerotinia sclerotiorum. I have three isolates from cucumber, sunflower and squash, can anyone advice on how to store this properly if I want...

29 April 2016 4,754 4 View

Is it possible to get different SSR allele size on individuals of the same cultivar?

I looked at allele size of SSR markers on williams 82 and compared it to the size on the same cultivar that were published on SoyBase. my question is, even though both are williams cultivar (the...

01 January 1970 2,723 10 View

What is the meaning of double bands on SSR marker PCR product?

I did a normal single marker PCR for my SSR markers and analysed the PCR product on a 3% agarose gel. two of the markers show two bands on the gel, the bands are very close together but there is...

01 January 1970 2,002 10 View

How long can I keep lyophilized primers before re-suspending?

I received fluorescent labelled primers and I am not sure how to re-suspend them. The information sheet doesn't give instructions that are usually given with primers, so I decide to wait until the...

01 January 1970 4,971 10 View