14 Questions 30 Answers 0 Followers
Questions related from Peter Durcan
Hi I have recently thawed out a new vial from atcc of phoenix ampho cells and put them into a T75 flask but 24 hours later the majority of cells have not adhered and are in suspension. I was...
04 April 2017 3,438 2 View
I am planning on doing some work with phage display libraries and was looking into my options as regards consumables etc that would be required. I came across hyperphage and want to get some...
09 September 2016 5,814 3 View
Ive noticed that when im using the biorad mini system for western blot there tends to be a small amount of overflow into adjoining wells when im loading the samples even though im loading often...
08 August 2015 6,024 5 View
I was wondering what causes expressed sequence tags to typically be quite short and only cover a small portion of the gene?
04 April 2014 9,460 7 View
I want to design some taq man assays to distinguish between different splice variants of genes. However some of these splice variants have pretty small nucleotide differences such as an extra 47...
11 November 2013 5,772 2 View
I would like to do 3' and 5' race experiments in order to get the full length sequence of some splice variants which I have detected. How hard is it actually to do this (I have not done this...
11 November 2013 9,801 6 View
I am currently looking at some splice variants of human genes and I have obtained sequence data from a PCR product which I had cut out of a gel. I have blasted this sequence against the human...
08 August 2013 3,344 1 View
Our research group currently has some goat primary antibodies and we wish to use these to examine human skeletal muscle on western blot. Our current hrp conjugated rabbit anti goat secondary is...
07 July 2013 8,718 1 View
I'm doing some work on human genes looking at possible differences in alternative splicing and I came across one mRNA which appears to have a very short 5' untranslated region of only 13...
05 May 2013 327 7 View
I'm currently investigating some proteins that have not been previously investigated in some human tissue samples and there is not much information available regarding these proteins in the...
04 April 2013 8,945 18 View
I was wondering have many people experience of using the new chemi doc mp imaging system for western blots using the HRP chemiluminescent detection method. If so have they experienced any problems...
03 March 2013 5,664 3 View
I have been doing some cryosectioning on frozen human skeletal muscle samples. The initial samples I worked with were not embedded in oct and were fine to cut on the cryostat. However, I now am...
03 March 2013 2,500 14 View
I would like to overexpress a gene in cell culture model. I have never done this before and from what i have read so far it typically involves establishing a stably expressing cell line usually...
01 January 2013 7,511 7 View
I am currently going through my options for knockdown and overexpression of genes in cell culture. I am interested in the shRNA option but have some questions as have no experience of using this...
01 January 2013 7,210 4 View
