Pei-Ching Huang

5 Questions 5 Answers 0 Followers

Questions related from Pei-Ching Huang

Is it possible to use yeast (beadbeated) lysate as starting material for Tn5 reaction (ATACseq)?

Hi all, I've looked for many ATACseq protocols using yeast as materials but most of them prep spheroplasts or isolate nuclei and then start the transposition reaction. I'm wondering if it's...

12 November 2020 7,810 0 View

Is Covaris machine ME220 suitable for yeast chromatin shearing?

I've been looking for machines that shear chromatin for my ChIP seq experiment using yeast lysate and I wonder if anyone had tried to use Covaris machine to perform yeast chromatin shearing? p.s....

01 February 2019 5,314 2 View

Did anyone try to isolate gDNA from yeast using the methods: GC preps or Bust n Grab? Are these methods better than traditional zymolase+PK treatment?

Hi all, Recently I've been trying to test different genomic DNA extraction from yeast to quickly check genotypes of my transformed yeast by colony pcr. Since the traditional gDNA extraction took...

26 February 2017 2,954 3 View

Why am I getting PCR product of the size of my template?

Hi guys, i tried to amplify a drug resistant gene from a plasmid(around 3kb). The gel photo I got shoe two prominent bands of really big size, and a faint band(~1.3kb), which is the size of the...

21 June 2016 9,588 6 View

Why do I get a weird background on Southern blot and cannot detect ladder?

Got problems about Southern blotting... There are two problems for my Southern blot using 32-dCTP labeling. One is that there’s no ladder. The other is the huge weird background. (very clear black...

16 November 2015 2,213 3 View