Paula Suarez-Henriques

3 Questions 4 Answers 0 Followers

Questions related from Paula Suarez-Henriques

Will a immunofluorescence staining for nuclear proteins in ovarian histological sections, showing signs of tissue autolysis, work?

I cultured sheep ovary fragments for 7 days and after that they were fixated in Carnoy´s solution(ethanol 100%, chloroform and pure acetic acid 6:3:1) for 4 hours and conserved in ethanol 70%...

14 June 2019 7,249 1 View

How to choose among several transcript variant sequences for primer design?

There are 5 variants of the mRNA for the sheep phosphoinositide-3-kinase regulatory subunit 1 deposited in the NCBI nucleotide database. I would like to get a sequence amplified that is common...

15 April 2019 1,442 3 View

Why are there bands in my agarose gel of PCR for sheep ovary cDNA in the blank wells?

I am doing PCR for cDNA to test primers' temperatures and different concentrations of sheep ovary cDNA before I do qPCR. In this photo of the agarose gel, the lanes where there is less trace...

19 March 2019 7,853 7 View