Patrick Gleason

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Questions related from Patrick Gleason

Has anyone added bacterial tRNA with Rabbit Reticulocyte Lysate?

Hello, Has anyone added bacterial tRNA charged with an Unnatural Amino Acid to Promega's TnT Rabbit Reticulocyte Lysate System? I'm looking at the Cosmobio CloverDirect kit but my protein isn't...

04 September 2015 3,979 1 View

How much RNA can you retrieve through phenol chloroform extraction?

I am doing an experiment where I am adding RNA to Promega's TnT Quick Coupled System (rabbit reticulocyte lysate) and then performing phenol/chloroform extraction. I started with a final...

20 April 2015 9,822 3 View