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Questions related from Patricia Román-Carrasco
Hi! I want to deproteinize a sample and then apply the resulting deproteinized product to cells in culture (PBMCs). Which method can I use for this? I thought about TCA, but I am not sure whether,...
11 December 2023 3,523 4 View
I need to perform a blood draw from a patient and we are interested in keeping intact the VLDL present in the sample, because we want to do further analysis to them!... which would be the best...
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Hi! I am trying to develope a sandwich ELISA to detect a molecule in chylomicrons produced by Caco-2 cells. I normally coat the ELISA plate with anti-ApoB antibody, block with serum albumin, then...
19 September 2017 8,415 8 View
Hello! Quite often, when I ve done a TLC with immunostaining (I overlay with poly isobutyl methacrylate), I get in the picture those ugly huge bright/white areas instead a clean picture! Does...
27 July 2017 5,714 1 View
Hi! I wanted to perform ultracentrifugation to isolate chylomicrons and VLDL particles from culture media (colorless and low glucose DMEM). I have some questions regarding the procedure: - Could...
29 June 2017 8,638 5 View
I am not a "lipids" expert, and also nobody in my lab... but I need to perform a glycolipid extraction and fractionation. To remove the chloroform I can use a Speed vac device, but it is out of...
26 November 2015 5,165 12 View
