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Questions related from Patricia Román-Carrasco
Hi! I want to deproteinize a sample and then apply the resulting deproteinized product to cells in culture (PBMCs). Which method can I use for this? I thought about TCA, but I am not sure whether,...
11 December 2023 3,596 4 View
I need to perform a blood draw from a patient and we are interested in keeping intact the VLDL present in the sample, because we want to do further analysis to them!... which would be the best...
26 February 2018 4,938 5 View
Hi! I am trying to develope a sandwich ELISA to detect a molecule in chylomicrons produced by Caco-2 cells. I normally coat the ELISA plate with anti-ApoB antibody, block with serum albumin, then...
19 September 2017 8,488 8 View
Hello! Quite often, when I ve done a TLC with immunostaining (I overlay with poly isobutyl methacrylate), I get in the picture those ugly huge bright/white areas instead a clean picture! Does...
27 July 2017 5,757 1 View
Hi! I wanted to perform ultracentrifugation to isolate chylomicrons and VLDL particles from culture media (colorless and low glucose DMEM). I have some questions regarding the procedure: - Could...
29 June 2017 8,673 5 View
Hi! I want to perform a TLC with immunostaining but, when I tried to disolve the Polyisobutylmethacrylate beads in Hexane to coat the TLC plante, these were not disolving at all. I tried to crush...
24 May 2016 6,453 2 View
Hi! As I mention in a previous question I am not a "lipid expert", neither any college in my lab, but I came to a point in the research for my PhD thesis, where I need to extract and work with...
11 December 2015 7,739 2 View
I am not a "lipids" expert, and also nobody in my lab... but I need to perform a glycolipid extraction and fractionation. To remove the chloroform I can use a Speed vac device, but it is out of...
26 November 2015 5,200 12 View
