Parul Sahu

4 Questions 12 Answers 0 Followers

Questions related from Parul Sahu

What to do if we get higher ct value of house keeping genes in qPCR ?

I have reconstituted the RNA from RNA stable tube biomatrica catalogue number 93221001.The 260/280 was 1.81 while 260/230 was 2.The ct value for GAPDH is 27 .Will this value is acceptable.What...

08 August 2017 4,053 7 View

Does knockdown of particular gene expression in liver of mice through sirna and further maintaining its progeny possible?

I want to knockdown some gene expression particularly in liver of mice.can somebody help me to provide protocols and proper strategies as well refrences

02 February 2016 2,665 3 View

What is the procedure for detecting gfp contained parasite in mice blood?

I have injected mice intraperitoneal with gfp plasmodium berghei. I have to confirm the presence of gfp. Can anybody help with the appropriate method for the detection.

04 April 2015 2,709 3 View

What is the concentration of PBS solution used in MTT assay?

PBS concentration in MTT assay

07 July 2014 2,463 4 View