Paolo Tenti

4 Questions 31 Answers 0 Followers

Questions related from Paolo Tenti

Melting curves with the same shoulders but with different primers, possible reasons??

I am getting crazy about this. I am having less than perfect melting curves, with different primers. The funny thing is that while primers give a main peak at different temperatures, as it is to...

08 August 2018 9,091 13 View

How long Interphase and Phenol/chloroform phase can be store?

Anybody has experience and can advise on how long the DNA survive in the Interphase and Phenol/chloroform phase and at which temperature? Only mention I could find in Trizol manual was overnight...

12 December 2017 1,848 3 View

Thermodynamic Oligo Alignment vs Traditional secondary structure alignment, who is the winner?

This is an option in Primer-Blast to select which model to use to calculate the propensity of the primers to form secondary structure. I am puzzled about which option is better, the output of the...

11 November 2017 674 0 View

Long term storage and handling of RNA, what about 66 to 75% EtOH without Na Acetate?

HI, I know that RNA is ideally preserved long term after precipitation in EtOH and Sodium Acetate, and it is successively washed in 75% EtOH to remove salts before rehidratetion. I have also read...

08 August 2017 1,330 10 View