Pankaj Losan Sharma

4 Questions 3 Answers 0 Followers

Questions related from Pankaj Losan Sharma

Can anyone suggest protocols for successful conjugation of Ecoli (TP003) with bacteria such as bacillus and Burkholderia for successful recombination?

I have tried to perform conjugation of E coli having GUS transposon(TP003) with bacillus , Burkholder strains, what I have observed that after mixing both the antibiotic selective strains..with E...

07 March 2019 6,795 1 View

How can I able to perform RNA sequencing of a specific pathogenic bacteria from plant?

I am working on a pathogenic bacteria , i want to analyze its gene expression by RNA seq, but the main obstacle is that I want to study the gene expression of these bacteria while it is present on...

24 June 2016 440 2 View

What are the methods or protocols through which we can isolate bacterial mRNA from total RNA?

I want to isolate bacterial mRNA from bacteria, but because of the presence of rRNA , tRNA in the total RNA , isolation of bacteria is not that easy. I need some protocols that will give me a...

07 April 2015 3,482 5 View

How can I isolate bacterial RNA from plant or seedlings?

I want to isolate prokaryotic RNA from plant and from seedlings. Is there any method to isolate prokaryotic or bacterial RNA from plant/seedlings. And can we separate the prokaryotic RNA from the...

09 January 2015 4,810 4 View