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Questions related from Pablo Menéndez
Dear friends, I am planning to cryopreserve and generate a HEK293T cell bank, but I have a few doubts on how to achieve best results. I am using "Corning® CoolCell® FTS30 container" for the...
01 June 2023 8,540 2 View
Hi guys, I was wondering what effect would have a slight temperature increase in my cell culture media. The PID controller of our reactor didn't work as expected and the temperature process...
24 May 2023 292 0 View
Hello guys, I was wondering what is the right way of laying down a lid is when working inside a laminar flow hood, BSC or isolator. Normally, if a cap of a container needs to be laid down, I...
13 December 2022 4,286 3 View
Hello guys, I've always centrifuged my HEK293Ts using 15 or 50 mL centrifuge tubes. For these, 300G for 3 min at RT (during standard passaging) gives me great results. However, now we are...
12 December 2022 682 3 View
I was thinking whether using NaBu might enhance my transfection effciency during a lentiviral production in 293Ts despite the fact my thrid generation system uses plasmids with no SV40 promoter....
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