Pablo Llevenes

6 Questions 3 Answers 0 Followers

Questions related from Pablo Llevenes

Does anybody know what these bright red structures could be?

So I stained mouse JC cells with phaloidin (red) after treatment (right picture) and found that most of the cells generated this structure in the apices of the cells compared to control group...

04 December 2023 1,688 1 View

How does FBS interfere with lipofectamine when transfecting miRNA?

I keep seeing everywhere that we have to use optimem for the lipofectamine to take the miRNA into the nanovesicles. But why? How could FBS be jeopardizing this process? Also, when the complex...

15 November 2022 9,073 3 View

Does anybody know how to isolate exosomes from visceral or subcutaneous adipose tissue of mice?

Hello researchers! I am wondering how to isolate exosomes from adipose tissue surgically extracted from mice. I am guessing that I would need to digest the tissue and in some way obtain a liquid...

14 October 2022 4,542 1 View

How to isolate adipocytes from mouse mammary tumor?

Hi! I am starting my project on breast cancer on mice and I would like to isolate the adipocytes found in and surrouding the tumor to grow them on cell culture. Is there a way to do this? I...

13 September 2022 2,795 0 View

Why do you need to use a matching 10% fat diet when you use a 60% High fat diet in mice instead of just using normal chow?

I have been wondering why when using a model of high fat diet the control diet has to be the 10% fat instead of normal chow. Does anybody understand the rationale behind this?

01 February 2022 5,595 2 View

Does anybody know about the legitimacy of the company ubigene?

I am thinking of ordering some cells expressing luciferase from this company. I was not able to find any rating from anyone, so I am just wondering if they are scammers or a company that actually...

01 January 1970 4,031 3 View