Pabitra Bhagowati

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Questions related from Pabitra Bhagowati

Sequencing and species level identification of different bacterial strains using universal primers for the house-keeping genes?

Can I use universal primers of rpoB, gyrB, etc. for species level identification of bacterial strains? How much is the efficiency of the technique of using degenerate primers?

06 January 2016 5,274 6 View

Can the color of the DCPIP solution containing bacterial culture turn to its original color from colorless after a certain period of incubation?

DCPIP assay is performed for identification of hydrocarbon utilizing bacterial isolates. The color change from blue to colorless signifies the potential of the isolates to use hydrocarbon as...

10 November 2014 417 2 View

Why is the temperature of the culture medium maintained at 30°C rather than 37°C in microbial degradation of hydrocarbon studies (in vitro)?

The temperature of culture medium of hydrocarbon degradation studies by bacterial strains is maintained at 30 degree Celsius in most of the cases. Why is that?

21 October 2014 997 1 View