Osama Madkhali

4 Questions 10 Answers 0 Followers

Questions related from Osama Madkhali

How to avoid aggregation after centrifugation for GNPs?

I prepared gelatin nanoparticles using two-step desolvation method. I used centrifugation to isolate nanoparticles but the problem is that the nanoparticles get aggregated when I redispersed them....

18 November 2016 8,982 5 View

How can I purify gelatin nanoparticles using tow-step desolvation method?

Hi, I am preparing gelatin nanoparticles using two-step desolvation method, the size of nanoparticles were about 150 nm.When I come to purification step I centrifuge the nanoparticles at 12,000...

24 September 2016 2,595 7 View

How to prepare B-alanine calibration curve?

I'm working on cationic gelatin and would like to measure the free primary amines using TNBS method as following. One milliliter of gelatin or cationized gelatin solution (0.50 mg/mL) in phosphate...

12 March 2016 7,372 3 View

Why does gelatin get precipitated when I adjust pH to 5 to prepare cationic gelating using spermine?

I am trying to cationize gelatin using spermine. First, I dissolved gelatin in 1 M PBS (pH=5), then I add spermine, but when I adjust pH using 6N HCL the solution get precipitated and cloudy, this...

07 March 2016 4,555 2 View