Osama Al Dalahmah

5 Questions 13 Answers 0 Followers

Questions related from Osama Al Dalahmah

Anybody having issues with poor Agilent Bioanalyzer RIN reproducibility?

I am having issues with RIN values I am getting with my RNA samples I extracted from postmortem brains. I got RIN values on 2 machines and the results were somewhat close: all above 7 and within...

03 May 2018 995 6 View

What are the optimal culture conditions for propagating patient-derived glioblastoma cells?

I am trying to culture glioblastoma cells from biopsies (5-6 mm) taken from GBM patients. The tumor : background parenchyma ratio will be variable. Anyone with experience on culture conditions: 1)...

23 January 2017 9,715 3 View

What is the best route to inject post-natal mice with BrdU?

I am currently injecting mice at P3-7 with BrdU, I use a 50mg/kg dose (concentration at 5-10mg/ml). I noticed that after intraperitoneal injection of volume between 10-30 ul, the BrdU solution can...

25 November 2013 9,987 4 View

Is there a specific marker for quiescent neural stem cells in the adult brain?

I am looking for a specific marker that can distinguish between quiescent and activated stem cells in Subventricular zone in the adult brain. Preferably, a marker that is not expressed in transit...

30 August 2013 1,774 17 View

Is there a good antibody against B-catenin to use in IHC for mouse brain sections (PFA-perfused free-floating sections)?

I am trying to stain mouse brain sections (coronal) for B-catenin and look for nuclear localization of B-catenin. The brains were PFA-perfised and stained using the free-floating section method. I...

22 March 2013 5,637 5 View