Omer Faruk Kuzu

4 Questions 25 Answers 0 Followers

Questions related from Omer Faruk Kuzu

Why there is no protein translation from the plasmid?

I am dealing with a strange problem. I want to overexpress PIK3R4 protein. I have 2 different constructs. The first construct encodes naive protein with Puromycin resistance gene. I transfect this...

03 March 2017 3,197 6 View

Can anyone provide suggestions for the interesting Western data on autophagy, p62, LC3B , Akt and pEIF4eBP?

I got an interesting result of Western Blot analysis related to a compound that we are working on. The illustration of the Western data is attached in the link. It seems that the compound inhibits...

01 January 2013 683 4 View

Problem regarding the stable transfection of floxed GFP into a B16F10 mouse melanoma cell line.

I am trying to obtain a stably transfected B16F10 cell line of floxed GFP plasmid (http://www.addgene.org/8389/). The plasmid structure is pCMV-lox-GFP-lox-RFP. Thus it is expected to express GFP...

11 November 2012 6,130 10 View

Interpretation of Electron Microscopy data (Refrehsed Question)

I did some E.M analyses with a compound that I am working on. I will be happy to hear you interpretations on the data. Following informations about the compound might be helpful 1) This is an...

10 October 2012 410 17 View