Oleksandr Galkin

4 Questions 19 Answers 0 Followers

Questions related from Oleksandr Galkin

How to do a kinetic proliferation assay for the long term cell culture?

Hi all, I have a somewhat basic question. But as I did not do such experiments before I was hoping someone could provide me with a quick answer whether what I am thinking to do is the correct...

06 June 2018 2,711 3 View

Reliable tissue expression database?

I need to look for expression of several genes in different adult human tissues. There are some databases available (I have checked BioGPS, Protein Atlas and Illumina Body Map). The problem is...

09 September 2017 2,198 4 View

What types of mouse BM cells can adhere?

If I flush the mouse bone marrow and culture it overnight in DMEM in tissue-culture treated plates, some cells adhere and remain so after the washings. When I Accumax them they come off very...

07 July 2016 7,754 4 View

Why are my macrophages positive for both CCR7 and CD206?

I have stained mouse BM with F4/80, CD11b, MHCII, CD206 and CCR7. Cells were Fc-blocked. When gated on F4/80_hi/CD11b_med-hi, I see a population that is CCR7+/CD206+ double positive (see...

02 February 2016 1,847 6 View