Oleg Dobrokhotov

2 Questions 5 Answers 0 Followers

Questions related from Oleg Dobrokhotov

Where did I mess up with my qPCR primers design?

Hi there, I'm trying to design primers to detect CRISPR-mediated KO using qPCR (as described in this paper: A qPCR method for genome editing efficiency determination an... ). I designed a few...

03 November 2022 5,269 2 View

Non-specific binding on Western Blots?

In my current experiment I'm trying to detect cytokeratin 10 by western blot in whole cell lysate of A431 cells. Unfortunately there are strong signals from non-specific binding in my blots (see...

14 May 2019 6,382 6 View