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We are interested in setting up click chemistry for making oligos for qPCR, currently we make qPCR probes using traditional fluorophore adding protocols, does anyone knows pros and cons of click...
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Does anybody know a simple protocol for oligo desalting in 96 well filter plates using Sephadex g-50? detailed protocols will be very much appreciated. Thanks!
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We need to purify many oligos per day, however to buy a commercial automated oligo purification system is not an option since they are quite expensive, however we have a way to design and build...
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