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Questions related from Norelle C. Wildburger
1) If I have an intracellular molecular probe (647 nm) perfuse the trasgenic animal and paraffin embed the tissue will I be able to see the fluorescence with a confocal of fluorescence...
01 January 2020 3,893 3 View
I am interested in using Bielschowsky's silver stain on brain tissue that has been embedded in LR White and sectioned 200-500 nm thin. Is there a protocol for this? Does silver stain work on both...
03 March 2018 8,240 2 View
I am finding a number of peptides in brain that are truncated at either the N-terminus or C-terminus or both compared to the canonical sequence. What is the best way to determine if these are an...
09 September 2016 5,832 1 View
I am having consistent problems with mass accuracy on the Orbitrap Fusion (see attached powerpoint). The LC is fine and so is the standard. There is some sensitivity issues but that does not...
02 February 2016 5,438 5 View
Does anyone know if 5 M Guanidine will artifactually modify amino acids in your peptide sequence. 8 M urea will carbamylate your peptide, but what about guanidine?
11 November 2015 6,522 3 View
Hi Everyone, I would like to do Immuno-SEM (not TEM) on brain slices. The antibody labeling will need to be post-embedding for reasons I don't want to get into. However, I will need to omit the...
10 October 2015 9,009 5 View
