Noha Mohamed

4 Questions 3 Answers 0 Followers

Questions related from Noha Mohamed

I want to pick a q-PCR marker for corpus luteum. In literature, they are using Cyp11a1 or 3beta HSD, but they are not specific.Any suggestions,Thanks?

I am using whole mouse ovary for the qpcr analysis and I want to have a marker for each ovarian functional cellular structure. I chose markers for granulosa cells and theca cells but I am confused...

06 June 2016 1,372 1 View

Is there a method to check RNA quality without using noxious chemicals?

I was trying to check RNA quality on a denaturing gel but it did not work. Because I am pregnant, I could not tolerate using formaldehyde and formamide in the preparations. Can you suggest other...

03 March 2014 6,972 2 View

Can anyone help with fallopian tube epithelium isolation for culturing and FACS sorting?

I was wondering if anyone can help with this issue. I need a method to separate fallopian epithelium to quantify certain genes in the epithelial layer. If there is an enzymatic digestion method,...

03 March 2014 2,890 0 View

Why is rt pcr not amplifying b actin in all tissues?

I have been doing rt pcr from different mice tissue for b actin for 3 times now. Every time, certain tissues are amplified and others are not. everytime I do new cDNA. I quantified my dnase free...

02 February 2014 7,962 5 View