Nirali Rathwa

6 Questions 9 Answers 0 Followers

Questions related from Nirali Rathwa

RNA extraction from pancreas?

We tried RNA isolation from murine pancreas many times but we are getting degraded RNA. Kindly suggest precaution for the same.

12 December 2017 7,472 7 View

Does SNP in 3' UTR region affects over all protein function?

SNP in the 3' UTR region is involve in any kind of mechanism. These SNPs play any role in protein function, if yes how? How can one find that?

05 May 2017 2,330 6 View

What is the most crucial step for RNA isolation from human adipose tissue?

I am trying to isolate RNA from human adipose tissue but I am not able to get it. I have already tried from PBMCs and other cell type, from which I am able to isolate.

02 February 2016 7,054 4 View

Why am I getting lot of non-specific pcr band?

I have already tried decreasing primer concentration, Touch-down PCR and increasing annealing temperature. My pcr product is of 200bp. Gel image of my pcr product with two different dna samples,...

01 January 2016 9,772 8 View

Which step is most crucial for DNA isloation???

for plasmid dna isolation

01 January 2015 8,697 2 View

Can someone help me find the PENHAPLO program for haplotype & phenotype analysis?

PENHAPLO program for haplotype & phenotype analysis

01 January 1970 1,191 3 View