Nima Mohaghegh

7 Questions 14 Answers 0 Followers

Questions related from Nima Mohaghegh

Has anyone cultured THP-1 cells in Shaker-flasks?

I wonder if anyone has cultured THP-1 cells in shaker-flasks? If so, what is the appropriate shaking rate? And if you recommend any anti-foaming reagent being added to RPMI? Or, do you recommend...

13 June 2016 3,555 3 View

What is the optimal concentration for GM-SCF and/or IL-4 to differentiate human-PBMCs into MQs or DCs?

In literature, I see various concentrations for GM-CSF and/or IL-4 to differentiate hPBMCs into macrophages or DCs. What are the concentrations that you guys use? And what might be the reason for...

11 January 2016 5,360 4 View

Nuclear protein(s) that does not interact with chromatin?

Hi, I'm looking for a nuclear protein that does not interact with chromatin. Are importins a good option? And if so; which importin works best for Western analysis? Are there ones that are not...

14 September 2015 7,652 3 View

Wwhich viral vector has the best infection yield in primary CD14+ monocytes?

As I know, the susceptibility for transduction by a certain viral-vector differs from one cell line to another. Now, I'm going to try infecting primary CD14+ monocytes upon isolation from the...

08 September 2015 2,586 5 View

Which LPS is best for TLR4-only stimulation in THP-1 cells and do I need to add any other compound(s) to the culture (CD14 or..)?

For the time being I'm using the one from E.coli 0111:B4 by Sigma. I see different kinds used by different scientists: E. coli O111:B4, O55:B5, O157:H7, J5, K12, O113; Salmonella typhimurium,...

25 September 2014 9,709 3 View

Which protein(s) should I check for in my nuclear extract to make sure it is free from cytosolic contamination?

I am trying to use western blot to show the p65 translocation from the cytosol into the nucleus upon LPS induction in Jurkat, BJAB and macrophages. But I'm detecting Beta-Tubulin in my nuclear...

22 April 2013 2,986 23 View

Is there any evidence showing a role for bases flanking a binding sequence in interaction of a binding factor with its "non-DNA-binding" co-factor?

I know that certain protein-binding DNA sequences can determine the protein-protein interaction of the DNA-interacting transcription factor with its cofactor that does not interact with any DNA...

02 March 2013 6,708 4 View