Nima Mohaghegh

4 Questions 13 Answers 0 Followers

Questions related from Nima Mohaghegh

Has anyone cultured THP-1 cells in Shaker-flasks?

I wonder if anyone has cultured THP-1 cells in shaker-flasks? If so, what is the appropriate shaking rate? And if you recommend any anti-foaming reagent being added to RPMI? Or, do you recommend...

13 June 2016 3,514 3 View

Nuclear protein(s) that does not interact with chromatin?

Hi, I'm looking for a nuclear protein that does not interact with chromatin. Are importins a good option? And if so; which importin works best for Western analysis? Are there ones that are not...

14 September 2015 7,622 3 View

Wwhich viral vector has the best infection yield in primary CD14+ monocytes?

As I know, the susceptibility for transduction by a certain viral-vector differs from one cell line to another. Now, I'm going to try infecting primary CD14+ monocytes upon isolation from the...

08 September 2015 2,554 5 View

Which protein(s) should I check for in my nuclear extract to make sure it is free from cytosolic contamination?

I am trying to use western blot to show the p65 translocation from the cytosol into the nucleus upon LPS induction in Jurkat, BJAB and macrophages. But I'm detecting Beta-Tubulin in my nuclear...

22 April 2013 2,957 23 View