Nick Shih

6 Questions 1 Answers 0 Followers

Questions related from Nick Shih

Why did I get a collection of 5 genes when I search for the gene data of SMN1?

This is what I got when I searched for SMN1 (survival of motor neuron 1) gene: https://www.ncbi.nlm.nih.gov/gene/6606 However, when I downloaded the sequence data, I found there are 5 gene...

15 August 2023 8,150 0 View

What is an easy way to spike the blood for making the reference or standard samples for molecular genetics?

Is there an easy way to manipulate the amount of DNA in the blood cells so the reference blood would have the same amount of housekeeping genes but difference amount of target genes? In order to...

22 April 2020 9,014 0 View

How to extract DNA with Quantabio DBS buffer and Hamilton robotic system efficiently?

I am trying to solve the problems with extracting DNA in the dried blood spot (DBS) with the one-step Quantabio Extacta DBS buffer for qPCR reactions. https://www.quantabio.com/extracta-dbs...

13 February 2020 9,736 0 View

Spike DNA into the blood without being washed away?

In order to make the reference materials for genetic screening of blood, I want to spike synthetic DNA into blood. However, the spiked DNA can easily be washed away during the first step of DNA...

12 December 2019 1,980 0 View

What is the appropriate ratio of acrylic acid to styrene for polystyrene nanoparticle synthesis?

I am making polystyrene nanoparticles with carboxylic groups on the surface by emulsion polymerization of styrene and acrylic acid at the ratio of 4:1 or 20% acrylic acid (with the surfactant...

22 May 2019 6,169 0 View

How do I prevent protein conjugated polystyrene nanoparticles from aggregation over time?

I have synthesized ~150 nm polystyrene nanoparticles with carboxylic groups on the surface. I used EDC chemistry to cross-link the carboxylic group with protein. After the reaction, I used Tris to...

20 May 2019 5,652 3 View