Nicholas J Davies

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Questions related from Nicholas J Davies

Does anyone have a protocol for EBV transforming CLL cells?

Hi, I was wondering if anyone has a robust protocol for transforming chronic lymphocytic leukemia cells? If you do and you would be willing to share it, could you also tell me if you've ever tried...

07 January 2022 3,758 0 View

What is the minimum percentage of Matrigel you would recommend for generating prostate organoids?

I am about to begin a project where I will be generating organoids from prostate tissue. I have read several protocols that use growth factor reduced Matrigel. I was speaking to a rep about it and...

30 June 2020 948 0 View

Can genome editting technologies such as CRISPR/CAS9 be used on non-cycling cells?

Genome editting technologies, such as CRISPR/CAS9, rely on either non-homologus end-joining and homologous recombination. My understanding is that homologous recombination is specifically...

27 November 2015 2,828 3 View

What is the best anticoagulant to use to isolate and characterise exosomes from plasma?

I am hoping to study exosomes from patient samples and have read some literature saying that the blood shouldn't be collected in EDTA or heparin-containing tubes. I was wondering if anyone could...

31 July 2015 607 2 View

Has anyone got a protocol for lentiviral transduction of B-CLL cells?

I have been trying to transduce primary CLL cells with virus made using psPAX2 and pMD2.G plasmids. The virus I have generated works because I have managed to transduce HEK cells as well as the...

04 March 2015 8,153 8 View