Ngala Elvis Mbiydzenyuy

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Questions related from Ngala Elvis Mbiydzenyuy

What could be responsible for the non-amplication of a reference gene in a multiplex assay?

What could be responsible for the non-amplication of a reference gene in a multiplex assay. The the other two targets amplified but the third (my reference gene) didn't. It however showed...

14 September 2023 2,923 0 View

Assay multiplex qPCR volume issues. What volume of assay mix when the dye assay mix of 3 targets are mixed together?

I am using Taqman Assay Multiplex supplies the expression of 3 genes in my samples. The protocol says for each reach add: Master mix = 5 ul Dye Assay mix (Target 1) = 0.5 ul Dye Assay mix...

12 September 2023 7,768 3 View

How can we better foster partnership between basic medical scientists and physicians to better implement an integrated curriculum?

I am a junior faculty lecturing Physiology at the School of Medicine, Copperbelt University, Zambia. We make use of an integrated model curriculum. This has not been without great challenges in...

14 June 2020 9,721 0 View