Neslihan Ögel

4 Questions 3 Answers 0 Followers

Questions related from Neslihan Ögel

How and why show different databanks different miRNA-numbers and targets for a specific gene?

Hi everyone, I am trying to analyze the miRNA-Targets of the FOSL1 gene but I am facing a particular problem. With Targetscan 7.2: -> 2 different miRNA Targets for FOSL1 got predicted. The...

11 February 2019 2,729 2 View

How can I find out which regulatory elements the 3' UTR of a specific gene contains ?

I used CRISPR/Cas9 to cut the FOSL1 gene within the last Exon and 3' UTR. RT-qPCR and Western Blot Analysis revealed downregulation of mRNA and protein. How can I find out which exact regulatory...

01 February 2019 7,757 3 View

Yeast infection in HEK293 cells - how to handle?

Hi guys, unfortunately I have a yeast infection in my HEK293 cells, which I wanted to validate for a K/O. I don't have cells left without contamination, so decontamination would be the only...

10 October 2018 8,485 4 View

Which protein concentration is suitable for WB with FOSL1-antibody (FRA1- antibody)?

Hey guys, Since I don't have time or capacities for testing the FOSL1-antibody (also known as FRA-1) I wanted to ask those of you who already worked with this antibody. Could anybody recommend...

04 October 2018 6,985 1 View