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Questions related from Neerajha Nagarajan
We perform primary cardiomyocyte isolation from 2-3 day old rat neonates. We recurrently obtained a cell yield of ~20 Mil cells from a litter of 10 pups. But, since the last 6 months, the cell...
05 February 2014 8,565 3 View
Is it possible to use the di-8-anepps potentiometric dye to measure inherent membrane potential of cardiomyocytes? Is an applied external electric field necessary? What is the best way to quantify...
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Can someone suggest an optimum cell seeding density (& media volume) for seeding isolated nenatal rat cardiomyocytes on a 25mm coverslip? I have fibronectin micopatterns on the coverslips for cell...
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