Neerajha Nagarajan

4 Questions 4 Answers 0 Followers

Questions related from Neerajha Nagarajan

How is the adhesion strength of protein transferred?

How does the adhesion strength of a protein transferred from hydrophobic (untreated) PDMS stamp to untreated glass substrate compare to a protein transferred from a hydrophyllic (air plasma...

09 April 2014 5,246 1 View

How to improve cell yield from neonatal cardiomyocyte isolation?

We perform primary cardiomyocyte isolation from 2-3 day old rat neonates. We recurrently obtained a cell yield of ~20 Mil cells from a litter of 10 pups. But, since the last 6 months, the cell...

05 February 2014 8,590 3 View

Di-8-anepps for cardiomyocyte conduction potential?

Is it possible to use the di-8-anepps potentiometric dye to measure inherent membrane potential of cardiomyocytes? Is an applied external electric field necessary? What is the best way to quantify...

05 October 2013 7,202 2 View

Seeding density (/ml media) of neonatal rat cardiomyocytes for maximum confluence?

Can someone suggest an optimum cell seeding density (& media volume) for seeding isolated nenatal rat cardiomyocytes on a 25mm coverslip? I have fibronectin micopatterns on the coverslips for cell...

16 May 2013 1,866 2 View