Neeraj Sharma

7 Questions 7 Answers 0 Followers

Questions related from Neeraj Sharma

Any one have protocol to get DNA from the 138+ cells suspended in RNAlater solutions and preserve in -80?

I want to extract DNA from 138+ cells suspended and preserved in RNA later at -80. Any one have experience and protocols, plz share with me. My each sample have 1 million cells when preserved.

14 February 2020 7,086 0 View

What minimum amount of protein required in cell lysate for Mass Spectrometry?

I want to go for Mass Spectrometry for biotinylated protein present in cell lysate.

21 August 2018 6,480 3 View

How long do I need to treat transfected HEK 293 cells with Brefeldin A and the concentration need to see endo H resistance in glycosylated protein ?

I am using knockout HEK 293 cell.

30 July 2018 3,122 2 View

I want to ligate my 1kb insert (PCR product) with 6 kb vector, both are digested by ECORV (creating blunt end) and Sal1 (creating sticky end). How?

I want to ligate my 1kb insert (PCR product) with 6 kb vector, both are digested by ECORV (creating blunt end) and Sal1 (creating sticky end). How? Is blunt end may create problem in cloning?

26 June 2018 4,288 3 View

Why Polyst. Flat bot. high Bind. MP (Costar) blank micro-plate is reactive with positive control?

I am using Polyst. Flat bot. high Bind. MP blank (Costar) micro-plate for making ELISA (HIV ), when i am using this blank plate for ELISA assay , it is found reactive with positive control while...

19 January 2017 2,708 5 View

What are the good microbial genetic targets to study the microbiome of lower respiratory track in asthmatic subjects?

I would like to identify the difference of microbiome in asthmatic and non asthmatic subjects. Which available technique is most efficient for this study.

24 July 2015 8,171 4 View

One SNP is reported C>A variation in NCBI, in a paper from China population. I got A>C variation in Indian population. Can someone help me to explain why?

Gene=FCER2:rs7249320:

28 May 2013 2,424 3 View