Navina Chrobok

4 Questions 12 Answers 0 Followers

Questions related from Navina Chrobok

Can anyone help with obtaining mouse blood after sacrification?

Hello! We are trying to obtain mouse blood to isolate PBMCs and do some tests. We do need the maximum amount of blood we can get but cannot use the mice as long as they are alive. We usually do...

05 September 2014 8,898 4 View

Has anyone performed adhesion and/or transendothelial migration with monocyte/macrophage cells lines (mouse, rat)?

I am interested in testing several chemical compounds that might have an effect on endothelial adhesion and/or transendothelial migration of monocytic cells. Since the ultimate aim is to test the...

16 July 2014 1,449 2 View

What Cytokines receptors are expressed by RAW264.7 and J774A.1 in mouse macrophages?

Does anyone know which cytokine receptors are expressed by these cell lines? I would like to do PCR on them to check the expression of several receptors to get an idea if cytokine treatments do...

14 July 2014 8,647 6 View

Can anyone help with PBMC isolation from mouse blood?

I would like to isolate PBMCs from mouse blood (cardiac puncture). I have found various different protocols and was wondering if anyone here can give me some tips which procedure works best. Is it...

15 April 2014 1,158 23 View