Natália Ferreira Mendes

3 Questions 2 Answers 0 Followers

Questions related from Natália Ferreira Mendes

Does anyone have a good protocol for skeletal muscle immunofluorescence in frozen tissues?

Is there any protocol with free-floating frozen slices? I have never done IHC in skeletal muscle so I would like to confirm if there is any secret during the frozen or in another step.

05 May 2018 2,108 1 View

What is the recommended dose of insulin to perform insulin tolerance test in mice?

What is the recommended dose of insulin to perform insulin tolerance test in C57BL/6JUnib mice (fed on chow or high-fat diet)? I have found 0.1 to 10 UI/kg in the literature. Which is an adequate...

02 February 2017 911 3 View

To study reward system, what is the best antibody to show dopaminergic neurons in immunohistochemistry?

Studies show two types: dopamine-beta-hydroxylase and tyrosine hidroxylase.

06 June 2014 4,772 2 View