Narendra Singh Yadav

10 Questions 17 Answers 0 Followers

Questions related from Narendra Singh Yadav

Is it possible to do RNA-Seq analysis (transcriptome) with one biological replicate to compare the two conditions?

How many minimum biological replicates are required to compare the transcriptome of two conditions?

24 November 2020 312 5 View

What are the best guidelines to use bleach (sodium hypochlorite) as a disinfectant at home?

04 April 2020 2,192 3 View

Can hydrogen peroxide priming in seeds causes change in plant genome sequence which we can identify by molecular markers e.g. RAPD, ISSR ?

Is there any reports that Hydrogen peroxide (H2O2) pre-treatment in seeds can cause changes in genome sequence? Can H2O2 works as mutagen?

18 March 2019 5,597 2 View

How we can be ensured that methyl jasmonate treatment works in plants? What are the physiological and molecular markers for MeJA response activation?

My question is that how it can be ensured that methyl jasmonate treatment works well? How we can demonstrate the activation of methyl jasmonate response at morphological, physiological and...

13 December 2018 4,232 3 View

Can I isolate gDNA for methylome sequencing from stored grinded leaf tissue which thawed at +2°C due to failure of our -80°C deep fridge?

Our -80°C deep fridge stopped working, temperature came to +2°C, my grinded leaf tissue samples are thawed which I have stored for Methylome sequencing. When I saw +2°C temperature, I immediately...

02 May 2018 5,986 2 View

How much minimum enrich ChIP DNA is required to proceed ChIP-seq?

27 April 2018 8,465 5 View

Why two guided RNA (gRNA) are used in CRISPR system to knockout single gene?

Why two guided RNA (gRNA) are used to develop single CRISPR mutant line?

26 April 2018 9,393 6 View

When Journals Impact Factor for 2020 will release?

In general, Journals Impact Factor release in the fourth week of June month every year? Does the release of Journals Impact Factor will be delayed in 2020 due to existing COVID-19 Pandemic?

01 January 1970 7,301 14 View

When Journals Impact Factor for 2021(2022) will release?

In general, Journals Impact Factor is released in the fourth week of June every year?

01 January 1970 4,829 59 View

Can we get homozygous transgenic Arabidopsis plants in T2 generation via using flower dip transformation methods?

I am interested to know the whole procedure for segregation including population size for segregation analysis to get homozygous transgenic plants.

01 January 1970 4,504 7 View