Narawat Nuamnaichati

4 Questions 2 Answers 0 Followers

Questions related from Narawat Nuamnaichati

Why does the A260/280 ratio decrease after measuring RNA with NanoDrop and then diluting it to equalize the RNA concentration?

Why does the A260/280 and A260/230 ratio decrease (as shown in the attached image) after measuring RNA with NanoDrop and then diluting it to equalize the RNA concentration? How can this issue be...

07 March 2025 8,499 0 View

Using a 20- gauge needle fitted to a syringe for homoginizing lysate in mRNA extraction?

I have a question about using a 20- gauge needle fitted to a syringe for homoginizing lysate in mRNA extraction. 1. Can I use single syringe to homoginize multiple test samples by resuspending...

13 November 2019 4,274 3 View

Staining Dermal Papilla (DP) cells with crystal violet

We seeded 2,000 Dermal Papilla cells in 96 well-plate and treated the cells for 24, 48, and 72 hrs in order to check the proliferative effects of our compounds. Then we stained them with 0.05%...

01 January 1970 1,246 9 View

How to set the conditions on cell sense dimension program taking image from the fluorescence staining to prevent bias between each group of experim.

I do experiment to detect oxidative stress stress by using DHE dry. I work on inverted microscope i81 Olympus and take images by cell sense dimension program. However, I wonder which conditions...

01 January 1970 787 0 View

Narawat Nuamnaichati

Why does the A260/280 ratio decrease after measuring RNA with NanoDrop and then diluting it to equalize the RNA concentration?

Using a 20- gauge needle fitted to a syringe for homoginizing​ ​lysate in mRNA extraction?

Staining Dermal Papilla (DP) cells with crystal violet

How to set the conditions on cell sense dimension program taking image from the fluorescence staining to prevent bias between each group of experim.

Using a 20- gauge needle fitted to a syringe for homoginizing lysate in mRNA extraction?