Namita Kumari

3 Questions 4 Answers 0 Followers

Questions related from Namita Kumari

All cell lines,CEM, THP1 and 293T are not growing when seeded at low density, What could be possible reason?

We shifter to new lab 8 months back. Since then all the cell lines which includes CEM, THP1 and 293T grow only and only when we seed then at high density like 6X10 to5. This is a huge problem as...

08 August 2017 1,948 3 View

293T cells are not spreading to form monolayer. What is the reason and Solution to this?

When I infected 293T cells and MS7 cells with Lentivirus they lost their monolayer property and started growing on top of each other. They are under puromycin selection. My labmate did Crisper-...

09 March 2017 7,930 1 View

How to isolate protein using PBMC contaminated with RBC?

I isolated protein and it viscous and red, how to retrieve my protein?

15 June 2016 4,989 3 View