Nameera Baig

3 Questions 2 Answers 0 Followers

Questions related from Nameera Baig

Cause of lower Protein groups from HeLa cell lysate QC in mass spectrometry?

We have a Q-exactive Orbitrap mass spectrometer. I have noticed that the number of protein groups observed from 100 ng of HeLa whole cell lysate (purchased from Thermo) has gone down from 1800 to...

27 September 2022 6,758 3 View

What causes hydrolysis of TMT labels?

How does TMT labels become hydrolyzed and lose their efficiency? We add 41 uL of the 800 ug TMT label to 100 ug protein in 100 mM TEAB buffer. Does the water in the TEAB buffer cause hydrolysis?

23 June 2022 7,412 0 View

What are the best methods for cleaning silver filter membranes in order to get rid of organic and inorganic particulate from the membranes?

I am encountering challenges with interference from particulate sloughing off from silver membranes that are interfering with my analysis.

03 April 2018 7,696 2 View