Nair Temis Olguin Alderete

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Questions related from Nair Temis Olguin Alderete

Has anybody tried the EasyPure RNA Kit (TRANS) for extracting rna directly from wine?

Hello, I have used Roche RNeasy kit for RNA extractions directly from wine with no problem at all but I am trying this other kit and I am having some difficulties. Has anybody tried the EasyPure...

01 January 2018 7,620 3 View

Is there a difference when extracting RNA from different varieties of wines?

Hello, I have extracted RNA directly from Cabernet Sauvignon with no difficulties but now I am trying to extract RNA from Pinot Noir and I can't do it. One possibility is due to the use of a...

01 January 2018 627 2 View

How do I choose among L-ornithine, poly-L/D-lysine or laminin coating?

I have used poly-L-lysine coating when culturing primary neuronal cells. Now I am going to start working with stem cells and I have a protocol indicating to use L-ornithine coating. So, is there a...

02 February 2016 816 2 View

N2a cell line culture with or without antibiotics?

Hello all, I am starting to work with N2a cell line and I don't know if I should use antibiotics in the culture medium or not. Some papers mention the use and some others don't. Also, I don't know...

01 January 2016 6,075 4 View