Nadia Skauli

3 Questions 19 Answers 0 Followers

Questions related from Nadia Skauli

Tips for running basic proteins in Blue Native PAGE?

Hi,I have native plasma membrane extracts from mouse brain which I am running on BN-PAGE gels. We have tested several concentrations of DDM and Digitonin, and 1% DDM + 1% Digitonin in 1X Native...

08 August 2016 7,526 13 View

How can I edit a Kozak sequence in a Gateway primer?

Hi, I need some advice on primer design for Gateway cloning. I am redesigning a forward primer in order to insert a strong Kozak sequence. My primer is as...

08 August 2014 6,461 3 View

Why am I having problems with 293T transfection and would fibroblasts help?

I am wondering if fibroblasts are hard to transfect. I have been attempting to express a human protein in 293T-cells, but I can not find clear evidence of protein expression in wt or transfected...

07 July 2014 4,823 17 View