Nadezda Kachurina

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Questions related from Nadezda Kachurina

Why Incucyte changes focus on my cells during during the Scratch Wound scan type?

We use the Scratch wound assay with 2h interval. The first scan is usually on focus and demonstrates a normal wound. However, the next scans showed something else that has nothing to do with the...

21 June 2023 2,120 0 View

Why does a luciferase reading increases with in increasing G418 concentration in cells with constitutively produced nanoluc luciferase?

I transiently transfected HEK293 cells with nano-glo dual-luciferase reporter assay system to create cells with constantly produced luciferase. Then, I treated cells with set of concentrations...

04 January 2023 7,699 3 View

How to avoid non-specific binding of anti-GST antibody to actin in human podocytes?

Dear All, I should to fix my cells in 4%PFA, because other antibodies that I need as a counter staining do not work with methanol or methanol/acetone fixation. Anti-GST poly clonal Rb (Abcam)...

29 March 2019 5,153 0 View