Myungchul Kim

5 Questions 19 Answers 0 Followers

Questions related from Myungchul Kim

How many days do we need to detect adoptively transferred Treg in the tumor site in tumor-bearing recipient mice?

Hello, I've got a plan to isolate mouse splenic Treg (WT and KO of target gene) and inject these CD45.2 Treg into tumor-bearing CD45.1 mice. My question is how many days do I need to wait to see...

12 February 2021 8,407 1 View

Can I measure lung weight after formalin fixation?

Hi, I have simple quick question. Can I make a measurement of mouse lung to evaluate lung metastasis after formalin fixation? Is it not reliable? Thanks.

12 January 2021 3,275 3 View

Tail vein versus retro-orbital injection for experimental metastasis assay?

Hi, researcher. It's a bit urgent that I have to inject tumor cells intravenously in 10 days. I am now practicing tail vein injection with a success rate of around 50%. I am still don't have...

21 October 2020 3,687 15 View

Why did mouse Treg suppression assay work in a opposite way?

Hi, folks. I am a postdoc newly working at the Immunology field. I am trying to establish a Treg suppression assay from the mouse. However, I have a weird issue unsolved yet. Here is the...

27 September 2020 6,712 24 View

Can tail vein injection cause extravascular metastasis of tumor cells in black mice?

Hi, The aim of my study is to investigate the effect of the gene of interest on metastasis. I chose to experimental metastasis and have injected tumor cells (100,000 PY8119 or B16 cells) into...

01 January 1970 3,515 3 View