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Questions related from Murugadas A
If Mesenchymal Stromal Cells (MSCs) are used in cell therapy, what are the stromal cells and in what proportion they are recommended for cell therapy? I appreciate the guidance, reference,...
15 October 2020 548 2 View
Hi all, I have done qPCR with house keeping gene and GOI. My goal here is to check the expression of a particular gene and not to compare them with treated or untreated control. Is there a way...
13 October 2020 2,783 3 View
I am trying to isolate MSCs from intestinal biopsy. I was able to observe individual cells from five days. In tenth day I was able to see cells are group of cells (images attached). However, i...
17 August 2020 1,061 3 View
Hi guys, I have a strange problem, my human intestinal organoids are dying immediately next day after passage. Problem 1: I isolate crypts from both ileum and rectum, they establish very good...
23 July 2020 7,992 4 View
I am having a set of primers for a gene. I performed qPCR a week before and got anticipated results. But this time with another sample (same species), did in technical replicates each well shows...
16 September 2019 6,185 4 View
Nanodrop says the 260/280 value of my DNA sample is around 2.1 and the 260/230 value is 2.3 and 2.4. Does it mean my sample is pure enough or contaminated?
13 July 2016 1,843 7 View
Hi all, I am working with an animal model called hydra. I can isolate and quantify protein from it. What i wanted to know is all how much quantity of protein will be required to perform a CYP...
01 June 2015 922 2 View
Can anyone suggest me the way to do LT50 (Lethal time 50) calculation?
30 March 2015 5,899 4 View
Could anyone help me with the protocol to prepare sample for ICP-AES analysis?
30 January 2015 4,227 3 View
Hello could anyone clarify my doubt here I am working with invertebrates to assess the toxicity of chemicals. Question 1 I exposed 25 animals to a toxicant (5 different doses) and my study period...
19 September 2014 7,108 6 View
