Murugadas A

7 Questions 14 Answers 0 Followers

Questions related from Murugadas A

How to calculate CT value with house keeping and GOI, without having any control for comparison? Is there any way other than absolute quantification?

Hi all, I have done qPCR with house keeping gene and GOI. My goal here is to check the expression of a particular gene and not to compare them with treated or untreated control. Is there a way...

13 October 2020 2,762 3 View

Problem with human intestinal organoid culture?

Hi guys, I have a strange problem, my human intestinal organoids are dying immediately next day after passage. Problem 1: I isolate crypts from both ileum and rectum, they establish very good...

23 July 2020 7,987 4 View

What could be the reason for different melting peaks within a sample?

I am having a set of primers for a gene. I performed qPCR a week before and got anticipated results. But this time with another sample (same species), did in technical replicates each well shows...

16 September 2019 6,178 4 View

How to check DNA purity by nanodrop?

Nanodrop says the 260/280 value of my DNA sample is around 2.1 and the 260/230 value is 2.3 and 2.4. Does it mean my sample is pure enough or contaminated?

13 July 2016 1,838 7 View

How much protein will be required to perform CYP and Caspase 3 assay?

Hi all, I am working with an animal model called hydra. I can isolate and quantify protein from it. What i wanted to know is all how much quantity of protein will be required to perform a CYP...

01 June 2015 917 2 View

How do I perform LT50 (Lethal time 50) calculations?

Can anyone suggest me the way to do LT50 (Lethal time 50) calculation?

30 March 2015 5,894 4 View

Can anyone advise on standard protocol for lethal concentration calculation, acute toxicity testing?

Hello could anyone clarify my doubt here I am working with invertebrates to assess the toxicity of chemicals. Question 1 I exposed 25 animals to a toxicant (5 different doses) and my study period...

19 September 2014 7,103 6 View