Muhammad Owais Shahid

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Questions related from Muhammad Owais Shahid

How to use Alpha Lattice Design in SAS?

I want to use Alpha Lattice Design in SAS. It is multi location and multi year trial. 4 locations, 2 years (4x2=8 environments) genotypes are 45. 2 replications 5 blocks/rep. Thanks in adcance.

08 January 2020 6,411 3 View

How to know that in which parts of a plant a specific genes expression is the highest?

I need to amplify a gene whose expression is very low in almost any cDNA I get. I want to know where or at what stage of a plant this gene is expressed the most. According the RNA sequencing...

07 August 2019 1,333 3 View

How to confirm protein translation of a cloned gene?

Its a real simple question, but i have no biotech base, and no one in my lab is free to provide me guidance. That's why i am asking this question here. I have been trying cloning a gene for...

06 June 2019 7,011 12 View

RNA extraction by pBiozol method?

I have tried so many times to extract tRNA from Rose leaves and buds by pBiozol method. My intention is to have tRNA for experiments about miRNAs (as pBiozol can give tRNA with high concentration...

21 July 2018 6,382 18 View

How to design specific primers for Stem-Loop RT and qPCR (SYBR Green)?

The primer designing for this kind of RT and qPCR are very simple. But how to check it specificity and quality, as it is used for miRNAs.

27 May 2018 9,172 3 View

Stem-Loop RT and qPCR (SYBR Green) analysis for miRNAs?

I have problem about the number of control samples in each of the steps of stem loop qRT. In the 1st step (Stem-loop RT), 2 types of controls are required [no RT control and no RNA control]. I am...

24 March 2018 6,610 3 View

How to differentiate between flower buds and vegetative buds?

I have problem collecting flower bud samples. I need to collect them just after the emergence of buds, which is really very tricky to differentiate from the leaf/vegetative buds.

12 December 2017 4,269 8 View

I m having trouble in primer designing?

I filtered 15 genes from Rosa hybrida degradome. I tried every possible field in primer3,5, ncbi, etc. May be there is problem in specifity. NCBI has no data for Roses, but we often match with the...

29 September 2017 4,805 18 View

Why is it so hard to extract total RNA from some tissue?

I work with Rosa hybrida tissues, and total RNA extraction from it is so hard. There are other plant species which gives good quantity and intact total RNA. So, what is the reason behind the Roses...

01 January 1970 5,634 2 View

Problem cloning a gene fragment

Hello experts, I want to clone a gene, which seems impossible to clone. First it is hard to amplify. After amplification (somehow) the vector is not ligating. I tried every possible vector...

01 January 1970 8,244 18 View