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Questions related from Muhammad Owais Shahid
I have tried so many times to extract tRNA from Rose leaves and buds by pBiozol method. My intention is to have tRNA for experiments about miRNAs (as pBiozol can give tRNA with high concentration...
21 July 2018 6,301 18 View
The primer designing for this kind of RT and qPCR are very simple. But how to check it specificity and quality, as it is used for miRNAs.
27 May 2018 9,126 3 View
I have problem about the number of control samples in each of the steps of stem loop qRT. In the 1st step (Stem-loop RT), 2 types of controls are required [no RT control and no RNA control]. I am...
24 March 2018 6,553 3 View
I work with Rosa hybrida tissues, and total RNA extraction from it is so hard. There are other plant species which gives good quantity and intact total RNA. So, what is the reason behind the Roses...
01 January 1970 5,584 2 View
Hello experts, I want to clone a gene, which seems impossible to clone. First it is hard to amplify. After amplification (somehow) the vector is not ligating. I tried every possible vector...
01 January 1970 8,195 18 View
