Muhammad Rizwan Shafiq

5 Questions 9 Answers 0 Followers

Questions related from Muhammad Rizwan Shafiq

why a mutant segregates into four distinct morphological classes of plants? Any explanation.

I mutagenized transgenic Arabidopsis thaliana carrying promoter:GUS construct with EMS, and I screened a mutant which appears a false positive for GUS expression later on. But when I sow the M3...

09 March 2016 9,870 15 View

Why does an ABA-insensitive mutant produces less ROS under abiotic stress?

An ABA-insensitive arabidopsis mutant (screened by myself) is that which can produce green cotyledons on MS supplemented with 3, 5 and 10 uM ABA. 4-week old soil grown arabidopsis plants were...

13 May 2014 6,225 4 View

How can one treat arabidopsis seeds on MS media supplemented by ABA for gene expression analysis?

Around 30 mg of arabidopsis seeds are required for RNA extraction. How can one treat the arabidopsis seeds on MS media supplemented by ABA for gene expression analysis? I have a problem with...

05 May 2014 4,352 2 View

Chlorophyll a and chlorophyll b contents - can anyone help?

4-week-old soil grown Arabidopsis plants brought under salt and drought stress for two weeks. Plants under salt stress with sodium chloride have less chlorophyll b content as compared to...

20 November 2013 9,579 33 View

How can I prepare Fluridone stock solution?

I wanna prepare MS plates supplemented with Fluridone 10 , 20, 30 uM and so on . How can I prepare Fluridone stock solution, (what's its solvent). Anything useful in application and handling of...

16 July 2013 9,192 2 View