Muhammad Safiullah Virk

4 Questions 4 Answers 0 Followers

Questions related from Muhammad Safiullah Virk

What is wrong with t-Vector or Fragment?

Hello, I am working on some gene manipulation experiments; many times I faced a problem, When i t-clone my GOI(Gene of Interest) if give many colonies. When i run Colony PCR from the middle...

31 March 2016 9,150 1 View

What is the best working concentration for HEPES for E. coli cells?

Hello Mates, I need to know about HEPES concentration to make E. Coli competent cells . Previously, I am using 0.1 M CaCl2, but now i want to use HEPES only, Thanks for suggestions.

19 February 2016 2,349 1 View

What happens to one allele of a gene when the other is Knocked out?

Hello, As we all know that most of the higher Eukaryotes are having couples of chromosomes so as called as DIPLOIDS, and each gene has an ALLELE also, may be homozygous or heterozygous. When we...

04 January 2016 6,179 1 View

From DNA to protein, many steps are necessary to make mature mRNA. Is it possible that antisense fragment of a sense gene is RNA without modification?

Hello Friends, In Eukaryotas, splicing is well known phenomenon and is used by almost every cell. During RNA synthesis, introns are removed and exons are joined to make mRNA, the actual size if...

21 December 2015 8,115 4 View