Muhammad Hassan Raza

4 Questions 8 Answers 0 Followers

Questions related from Muhammad Hassan Raza

How to align PDMS layers for fabrication of microfluidic devices?

Hello everyone, I am using multilayer soft lithography to fabricate microfluidic devices. I am struggling to align the PDMS layers using a stereo microscope since both of the layers stick...

08 July 2022 6,048 9 View

Does anyone have any tips on peeling off thin PDMS membrane from a 3D printed mold?

I have a 3D printed mold that I am using for transferring microfluidic features onto a thin PDMS membrane (~200 um). During the peeling-off process, either the features deform or the membrane...

08 June 2022 7,917 3 View

What is the recommended speed of centrifugation prior to resuspension of the oligos received from IDT?

I have received some oligos for my research from IDT in dried form. What is the recommended speed of centrifugation* prior to resuspension? *centrifugation prior to resuspension is suggested by IDT.

14 June 2021 9,427 3 View

How to find change in protein after identification of a SNP in DNA sequence?

I have recently identified SNPs in DNA sequences of some human genes responsible for a disease. I want to check if there is any change in the protein sequence as well as function. Are there any...

30 September 2019 2,200 3 View