Muhamad arif Budiman

5 Questions 3 Answers 0 Followers

Questions related from Muhamad arif Budiman

What is your opinion on coating antibody with shaking (ie on a plate shaker) ?

I am really looking to find out the coating antibody in microplate. I needed some advice, is it right to coating antibody with shaking (ie on a plate shaker) or not?.

25 March 2017 8,203 3 View

Serum or Plasma is the best way to detect folate via ELISA/HPLC?

in our lab we collect serum, but I don't know why. Is there any difference to be found while doing ELISA with Serum and Plasma?plasma was collected via EDTA vacutainer tubes or heparin...

26 January 2017 7,591 2 View

How do I use carbodiimide to attach an folic acid to a amine group of protein (avidin/BSA)?

I have both avidin and folic acid, but can not find a specific protocol with steps, can anyone help here, thanks in advance.

10 January 2017 7,231 1 View

Can I conjugate folic acid on avidin with carbodiimide (cross linker)?

I am looking to attach "folic acid" to avidin. What I have in mind is to use "carbodiimide". firstly, FA was dissolved in NaOH 1M with carbodiimide for 2H, and then avidin was added into the above...

19 October 2016 4,816 2 View

What is the binding capacity of DEAE cellulose for folate binding protein ?

Concentration of folate binding protein (sample) is 2,5 - 3 mg/ml after dialysis. I am going to use 12 mL DEAE cellulose 52 (by journal), my lecturer ask me about capacity matrix but i don't know...

19 June 2016 132 1 View