Mourad A M Aboul-Soud

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Questions related from Mourad A M Aboul-Soud

Global adoption of biotech crops is expected to continue to grow in the future, particularly in developing countries.

This is a quote by Dr. Clive James, Chair of ISAAA said in June 2012 at the USDA Crop Acreage Report. How far is this statement true in the light of the current rising anti-GM food campaigns,...

03 March 2013 5,842 2 View

What is the best way for extracting total/miRNA from human plasma?

Whole-blood samples were collected from human subjects with the aim of extracting circulating RNA (including microRNA) from them for transcriptional profiling. My first question is: does any one...

11 November 2012 1,842 27 View

Real-Time Quantitative PCR (RT-qPCR)

What is the best excel-based simplified way or freely-available sofrware-based method for the calculation of RT-qPCR effeciencies in relative quantification of gene expression?. I normally use a...

11 November 2012 1,731 2 View

One of the most efficient and time saving cloning strategies that I ever used is the Invitrogen Life Technologies Gateway cloning technology.

Gateway technology is a universal platform that enables you to shuttle your gene of interest (GOI) into as many expression and functional analysis systems as you‘d like. What do you think?

08 August 2012 8,445 0 View

Do you think that Digital Droplet PCR (DDPCR) will eventually replace real-time qPCR technology?

Digital Droplet PCR (DDPCR) is a third generation PCR

08 August 2012 2,640 22 View