Molly Resnick

7 Questions 4 Answers 0 Followers

Questions related from Molly Resnick

Would SYBR Green dye interfere with pyro sequencing?

DNA was amplified using a PCR mix that included SYBR green dye. Could this DNA amplification undergo pyro sequencing without interference from the dye?

01 February 2016 9,642 4 View

What is causing these Taqman probes with the same sequence but different dyes to perform differently?

We are having a perplexing issue with some IDT double-quenched probes. We have a particular amplicon with a SNP and have control DNA that is wild-type, heterozygous, and homozygous for the SNP....

11 January 2016 1,552 2 View

How can I improve an allelic discrimination identification of SNPs?

We are trying to identify single nucleotide polymorphisms (SNPs) using allelic discrimination software on the Roto-Gene 3000. Each reaction includes a wild type probe, mutant probe, and DNA...

17 August 2015 4,901 6 View

How can I design probes in a GC poor region with a high enough Tm?

I am trying to use qPCR to genotype single nucleotide polymorphisms (SNP). Some of the SNPs are located in GC poor regions and I cannot get a probe Tm 8-10 degrees higher than my primer Tm. Do you...

25 June 2015 4,099 3 View

Any suggestions on how to decrease non-specific binding of Taqman probes?

I am trying to genotype SNPs by using two multiplexed probes: one with the mutant DNA sequence (HEX - 3BHQ_1) that fluoresces on the green channel and one with the wild type DNA sequence (6-FAM -...

08 June 2015 2,558 6 View

What are the alternate loci?

I am currently designing a SNP genotyping assay for CYP2D6 and I am confused about the alternate loci that keep appearing in my blast searches. What is an alternate locus? Does every gene have an...

13 March 2015 4,858 3 View

What does the allele CA|CA in the 1000 Genomes Browser mean?

Hi, I am trying to find homozygous mutant DNA for CYP2D6*6. SNPedia, ARUP, and the literature says this SNP is a T deletion at nucleotide number 1707 (according to M33388 sequence). When I go onto...

14 January 2015 9,380 1 View