Mohammed Aliyu-Paiko

11 Questions 33 Answers 0 Followers

Questions related from Mohammed Aliyu-Paiko

What phytobiotics would be the most effective in the tropics?

As alternative to antibiotics, fish nutritionists are exploring potential candidates to boost immune system of test animals and better prepare them to withstand opportunistic pathogens....

07 July 2018 4,106 2 View

What are the quickest ways to get a Host for a Humboldt Fellowship?

I want to apply for Humboldt fellowship but my immediate challenge is to search for a host. This is as my Postgraduate studies was in Asia and most of my referees are not Humboldt fellows. What...

04 April 2016 4,251 0 View

Number of publications, citations, H-index, i10 index; which is the most important index to identify researcher quality?

You are are about to hire a new researcher into your laboratory from a developing country that you have no references from. Your objective is to recruit a high quality researcher for a...

07 July 2015 2,794 0 View

Any ideas of a symposium scheduled to address issues on publication sharp practices?

The era of impact factor, h-index, g-index and open access has brought about several issues relative to sharp practices by researchers, scholars, journal editors, reviewers et cetera....

07 July 2013 9,768 2 View

Do you have your ORCID number, what do you think of it?

Since I joined this forum following on the hot trails of discussions about publication, journal quality, impact factor and h-index, please permit me to start a new(?) round of discourse on...

06 June 2013 3,687 34 View

Does pathogen challenge induce time-dependent regulation of genes for pro- and anti-inflammatory cytokine production? How best is this measured in Fish?

We are testing a dietary supplement for its ability to enhance immune response in fish, upon challenge with a specific pathogen. The mechanism we chose to monitor this effect is through up- or...

05 May 2013 2,161 4 View

Which is better to infect fish with probiotic organisms; probiotics mixed in feeds, added to the culture water or injected IP)?

We are trying to choose the best approach to deliver probiotic bacteria into the gut of small fish (2g, 2-3 inches) and in the literature, several methods are reported. Has anyone tried (or has a...

03 March 2013 229 24 View

Is it posible to determine (quantitatively) the differences in certain protein concentration in particular tissues (liver or intestine) of different treatments using SDS-PAGE only?

I used Biorad's RNA/Protein extraction kit and obtained both the RNA and the Protein isolates, simultaneously. However, it looks like the integrity of the RNA is is not intact, so I cannot use it....

01 January 2013 7,726 13 View

Will protein isolated with Nucleospin RNA/Protein kit work when used in ELISA?

I isolated both protein and RNA from fish intestines and liver, using Biorad's Nucleospin kit and would like to use the protein isolate to run ELISA for some cytokines (TNFa, IL1b and IL6). Is it...

01 January 2013 7,589 8 View

Any ideas on the best approach to challenge a Marine fish species with a vibrio at minimal handling stress? Dipping or IP injection?

I am trying to challenge Asian sea bass with Vibrio alginolyticus and would be measuring the release of cytokines over a time course within 48 hour period. I am trying to reduce other stressors to...

12 December 2012 1,814 11 View

extract always show only 1 band on a 1% Agarose gel?

always seems to appear as attached. I am told running a denaturing gel electrophoresis may not change my

12 December 2012 2,218 12 View